High antioxidant and DNA protection activities of N-acetylglucosamine (GlcNAc) and chitobiose produced by exolytic chitinase from Bacillus cereus EW5
نویسندگان
چکیده
Chitin-degrading bacterial strains were screened and tested for their ability to degrade shrimp-shell waste (SSW). Among the potential strains, B. cereus EW5 exhibited the highest chitin-degrading ability compared with other strains and produced 24 mg of reducing sugar per gram of dry SSW after 4 days of incubation. A TLC analysis of SSW biodegradation revealed that the chitosaccharides produced in the culture supernatant were mainly N-acetylglucosamine (GlcNAc) and chitobiose due to the isolate's exolytic chitinase activity. The culture supernatant exhibited a high degree of antioxidant activity, as indicated by 83% DPPH, 99.6% ABTS, 51% hydroxyl radical scavenging activity and 0.34 reducing power. The formation of GlcNAc and chitobiose during biodegradation of SSW is considered to be the major contributor to the antioxidant activity. The EW5 culture supernatant also displayed inhibition of DNA damage, enhancing the reutilization value of SSW. This report presents the first description of fermented production of GlcNAc and DNA protective activity of culture supernatant from SSW by B. cereus.
منابع مشابه
Chitinase system of Bacillus circulans WL-12 and importance of chitinase A1 in chitin degradation.
Bacillus circulans WL-12, isolated as a yeast cell wall-lytic bacterium, secretes a variety of polysaccharide-degrading enzymes into culture medium. When chitinases of the bacterium were induced with chitin, six distinct chitinase molecules were detected in the culture supernatant. These chitinases (A1, A2, B1, B2, C, and D) showed the following distinct sizes and isoelectric points: Mr 74,000,...
متن کاملIdentifi cation of Inducers for Chitinase B (ChiB) Production in Bacillus
Chitin is the main component of exoskeleton (cuticle) of crabs. We consume large amounts of crabs and shrimps for food and as a result, enormous amounts of their shell wastes are produced every year. Although chitin is utilized in environmental technology and food medical industries, most of shell wastes are discarded by incineration and burying. Thus, reutilization of shell wastes is an import...
متن کاملPeptidoglycan N-acetylglucosamine deacetylases from Bacillus cereus, highly conserved proteins in Bacillus anthracis.
The genomes of Bacillus cereus and its closest relative Bacillus anthracis contain 10 polysaccharide deacetylase homologues. Six of these homologues have been proposed to be peptidoglycan N-acetylglucosamine deacetylases. Two of these genes, namely bc1960 and bc3618, have been cloned and expressed in Escherichia coli, and the recombinant enzymes have been purified to homogeneity and further cha...
متن کاملCloning, expression and purification of Bacillus cereus endochitinase in the Escherichia coli AD494(DE3)pLysS expression system.
A chitinase gene from Bacillus cereus was cloned and expressed in Escherichia coli. The purified recombinant chitinase had much higher (128 fold) specificity to pNP-beta-(GlcNAc)(3) than to pNP-beta-(GlcNAc), suggesting endochitinase. Thirty-three amino acids in the N-terminal were recognized and cut off during expression, which consequently made the M(r) not correspond to that predicted.
متن کاملChitinase Isolated from Water and Soil Bacteria in Shrimp farming Ponds
Chitinases have received attention because of their wide applications in the medicine, biotechnology, agriculture, waste management and industrial applications such as food quality enhancer and biopesticide. Excessive use of insecticides has led to several problems related to pollution and environmental degradation. In this study, isolation and identification of native bacterial strains with c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 3 شماره
صفحات -
تاریخ انتشار 2014